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This project is collaborated between The Forsyth Institute (TFI) and The Institute for Genomic Research (TIGR), and is funded by National Institute of Dental and Craniofacial Research (NIDCR)

Size of the P. gingivalis genome

The genome size of P. gingivalis strain W83 was determined by digestion of genomic DNA with Xba I (TCTAGA) and Spe I (ACTAGT) which previously had been found to yield a small number of large restriction fragments separable by pulsed field electrophoresis (Fraser and Duncan, 1992). Pulsed field gel electrophoresis of chromosome digests were carried out using a Bio-Rad CHEF DRIII with run conditions determined by the CHEF Mapper. Gels were run under a variety of conditions to resolve poorly separated bands. Fragment sizes were estimated by comparison with the mobility of lambda concatamers and low molecular weight markers. From the Xba I digests the genome size was calculated to be 2,176 kb, and 2,250 kb from the Spe I digests.

picture1.gif (12145 bytes) PFGE of Xba I- and Spe I-digested DNA from P. gingivalis strains. Lane 1, Lambda concatamers: 2, strain 381; 3, 33277; 4, A5B3; 5, A7436; 6, W83; 7, HG405; 8, ITA-1; 9, W50.

This page is created and maintained by Drs. Margaret Duncan, Floyd Dewhirst, and Tsute Chen, Department of Molecular Genetics, The Forsyth Institute .

Last modified on 02/20/2002

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